Establishment of direct competition ELISA method for chloramphenicol labeled with magnetic beads

Using the chloramphenicol (CAP) monoclonal antibody prepared by this laboratory, the dilution ratio of the coating antigen 1: 6 000 and the enzyme-labeled CAP monoclonal antibody 1: 8 000 is used as the working concentration to establish CAP direct competition El ISA (cdELISA) Method, cdEI ISA detection sensitivity reached 0.1 t ~ g / I. Immunomagnetic beads were prepared using the xMag isothiocyanate terminal magnetic particle $ -M5 CAP monoclonal antibody, and the immunomagnetic beads were used to enrich the CAP in the sample. After extracting the CAP adsorbed on the surface of the immunomagnetic beads by ethyl acetate, and then detecting by cdELISA, the detection sensitivity of the CAP can be increased by 50 times, that is, 0.002 g /

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