1. How to thaw the serum so as not to damage the product quality?
We recommend that you remove the serum from the freezer and put it in a 2-8 ° C refrigerator to melt it, then melt it at room temperature. But it must be noted that during the melting process, it must be shaken regularly and evenly.
2. The best way to save serum?
We recommend that serum be stored at -5 ° C to -20 ° C. However, if stored at 4 ° C, do not exceed one month. If you are unable to use up one bottle at a time, it is recommended that you aseptically dispense the serum into an appropriate sterilized container, and then put it back into the freezing.
3. After the serum is thawed, it is found that there are flocculent precipitates, what should I do?
There are many reasons for the appearance of precipitates in serum, but the most common reason is that the denaturation of lipoproteins in the serum is caused. Fibrin (one of the proteins that form blood coagulation) will also exist in the serum after the serum is thawed It is also one of the main causes of sediment. But these flocculent precipitates do not affect the quality of the serum itself. If you want to remove these flocculent precipitates, you can divide the serum into a sterile centrifuge tube and centrifuge it at 400g. The supernatant can then be added to the medium and filtered together. We do not recommend that you remove these flocculent sediments by filtration, as it may clog your filter membrane.
4. Why heat inactivate serum?
Heating can inactivate the complement system. The activated complement participates in the lysis of cells, stimulates smooth muscle contraction, cells and platelets release histamine, and activates lymphocytes and macrophages. In immunological studies, heat-inactivated serum is recommended when culturing ES cells, insect cells, and smooth muscle cells.
5. Is it necessary to do heat inactivation?
Experiments have shown that heat-inactivated serum that has been properly processed is unnecessary for most cells. The serum after this treatment only slightly promotes the growth of cells, or has no effect at all, and even because the high temperature treatment affects the quality of the serum, the cell growth rate is reduced. In the heat-treated serum, the formation of precipitates will increase significantly. These precipitates are observed under an inverted microscope, such as "small black spots", which often makes researchers mistakenly think that the serum is contaminated, and put the serum in 37 In the ℃ environment, this sediment will increase more, which makes the researchers mistakenly believe that it is the division and expansion of microorganisms. Therefore, we recommend that you do not need to do heat treatment if not necessary. In this way, not only save your precious time, but also ensure the quality of serum!
6. Why does the fetal bovine serum stored in the refrigerator precipitate?
GIBICO fetal bovine serum is not pre-aged. When stored at 2-8 ° C, various proteins and lipoproteins in the serum (such as cold agglutinin, fibrinogen, vitronectin, etc.) may aggregate to form a precipitate or visible turbidity . This should not affect the quality of the serum. It is recommended to store fetal bovine serum at -20 ° C to avoid repeated freezing and thawing.
Office Workstation,Work Station For Office,Standing Workstation,Workstation Table For Office
Feat Top International(China) CO.,LTD , https://www.nbfeattop.com