Complete medium "Complete medium" refers to a medium that has been supplemented with various additives and contains all the ingredients of the medium, which can meet the needs of a specific cell growth. Usually, it is prepared with a limited composition medium. Some of the ingredients (such as glutamine) are added before use, and various supplements such as serum, growth factors or hormones are also added. There are many varieties of limited ingredient media, ranging from simple media (containing only (Essential amino acids, vitamins, inorganic salts) to complex complete media, up to various serum-free media. Complex media contains a large number of different amino acids, including non-essential amino acids and additional vitamins, and often add some special metabolites (Such as nucleoside, intermediate products of tricarboxylic acid cycle, lipids) and minerals. In general, F12 has a low concentration of nutrients (most suitable for clonal culture); and Dulbecco's modified Eagle's MEM (DMEM) [ D Burning Giant F World, 1959; Morton, 1970] The nutrient concentration is high, suitable for high cell density, large-scale reproduction. Barnes and Sato [1980] invented F12 and DMEM as 1: 1 mixture as a serum-free culture Base liquid, this Fang incorporates the characteristics of F12 with diverse nutritional ingredients and high DMEM nutritional concentration.Although this scheme is not completely reasonable, it provides an empirical formula that can be used as a basic medium, adding various special substances to meet Many different cell types are needed.
Bathroom Furniture,Bedroom Set Funiture,Bedroom Funiture Sets,Modern Bedroom Funiture
DUOBUY TECHNOLOGY SHANDONG CO., LTD. , https://www.dbtfurniture.com