Experimental principle: This kit uses the double antibody sandwich method to determine the level of canine histamine (Histamine) in the specimen. Microporous plates were coated with purified canine histamine (Histamine) antibody to make solid-phase antibodies. Histamine was added to the monoclonal antibody-coated microwells in turn, and then combined with HRP-labeled histamine antibodies to form antibodies -Antigen-enzyme labeled antibody complex, after thorough washing, add substrate TMB to develop color. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the histamine in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of Histamine in the sample was calculated by a standard curve.
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