The research on dye decolorization and biodegradation of aniline pollutants in printing and dyeing wastewater undertaken by the Guangdong Institute of Microbiology is an applied basic research project that the Guangzhou Municipal Science and Technology Bureau has established for the national “863†project. This project has completed the contents of all scheduled research projects, published four research papers and applied for two invention patents. It has been the first time in the world that Shewanella with broad-spectrum decolorization capability has been used to conduct printing and dyeing using genetic engineering-degrading bacteria. Wastewater treatment research has achieved good results.
In the Pearl River Delta of Guangdong, the printing and dyeing industry is developed, there are many small and medium sized printing and dyeing plants, the discharge of printing and dyeing wastewater is large, the wastewater treatment is difficult to decolorize, and aromatic contaminants such as aniline produced by decolorization after printing and dyeing are difficult to meet standards. Therefore, there is an urgent need for efficient and economical biological treatment of high technology and technical approaches and theoretical guidance that can improve the efficiency of existing wastewater treatment systems. In response to these problems, researchers from the Guangdong Institute of Microbiology designed a new type of anaerobic baffled reactor (ABR) combined with anaerobic-aerobic biological treatment and a sequencing batch aerobic activated sludge aeration reactor (SBR ) Combined new process, construction of a laboratory-scale treatment system, development of efficient decolorizing bacteria, aniline degrading bacteria, selection and application of genetic engineering bacteria, community structure, quantity and distribution of activated sludge microorganisms, biological treatment of printing and dyeing wastewater Study on the Key Factors of Decolorization and Degradation Efficiency. More than 500 strains of microorganisms with the function of dye decolorization and aniline degradation have been isolated from the natural ecology and the microorganisms of existing wastewater treatment systems, and the “Swarovski strain†with its broad-spectrum decolorization capacity and its plasmids have been found internationally. The azo dye oxidoreductase gene was used to construct an aromatic degradation engineering strain using the benzene ring cleavage oxygenase gene. In the combined application of ABR-SBR and high-efficiency decolorizing bacteria, the added strain can maintain long-term stability, genetically engineered strains It can strengthen the decolorization efficiency of decolorizing bacteria and increase the efficiency of aniline degradation, which lays a good foundation for the application of microbial high-tech in wastewater treatment, and also provides a theoretical basis for effectively controlling the normal operation of the treatment system and improving the treatment efficiency of printing and dyeing wastewater.
The ABR-SBR anaerobic-aerobic biological treatment process designed for the dye decolorization of dyeing wastewater and the degradation of aniline pollutants, acclimates and culturing microorganisms in an environment suitable for each environment in the reaction chambers to make the anaerobic flora They can grow under their optimum environmental conditions, give full play to anaerobic decolorization and degradation, and decolorize the dyes in the process of printing and dyeing wastewater, biodegradation of aromatic compounds such as aniline, and ammonia nitrogen removal in ABR-SBR anaerobic- The aerobic treatment process took place in an orderly manner, so that the printing and dyeing wastewater treatment effect and the stability of the operation were improved, and the overall treatment effect was good. The chromaticity, COD, aniline and other indexes of the treatment system effluent reached the level of the national comprehensive sewage discharge. standard.
The result is to improve the efficiency of wastewater treatment and reduce harmful substances in wastewater. In the treatment of printing and dyeing wastewater, it will become a high-tech printing and dyeing wastewater treatment technology that is economical, practical, safe and has good market prospects.
In the Pearl River Delta of Guangdong, the printing and dyeing industry is developed, there are many small and medium sized printing and dyeing plants, the discharge of printing and dyeing wastewater is large, the wastewater treatment is difficult to decolorize, and aromatic contaminants such as aniline produced by decolorization after printing and dyeing are difficult to meet standards. Therefore, there is an urgent need for efficient and economical biological treatment of high technology and technical approaches and theoretical guidance that can improve the efficiency of existing wastewater treatment systems. In response to these problems, researchers from the Guangdong Institute of Microbiology designed a new type of anaerobic baffled reactor (ABR) combined with anaerobic-aerobic biological treatment and a sequencing batch aerobic activated sludge aeration reactor (SBR ) Combined new process, construction of a laboratory-scale treatment system, development of efficient decolorizing bacteria, aniline degrading bacteria, selection and application of genetic engineering bacteria, community structure, quantity and distribution of activated sludge microorganisms, biological treatment of printing and dyeing wastewater Study on the Key Factors of Decolorization and Degradation Efficiency. More than 500 strains of microorganisms with the function of dye decolorization and aniline degradation have been isolated from the natural ecology and the microorganisms of existing wastewater treatment systems, and the “Swarovski strain†with its broad-spectrum decolorization capacity and its plasmids have been found internationally. The azo dye oxidoreductase gene was used to construct an aromatic degradation engineering strain using the benzene ring cleavage oxygenase gene. In the combined application of ABR-SBR and high-efficiency decolorizing bacteria, the added strain can maintain long-term stability, genetically engineered strains It can strengthen the decolorization efficiency of decolorizing bacteria and increase the efficiency of aniline degradation, which lays a good foundation for the application of microbial high-tech in wastewater treatment, and also provides a theoretical basis for effectively controlling the normal operation of the treatment system and improving the treatment efficiency of printing and dyeing wastewater.
The ABR-SBR anaerobic-aerobic biological treatment process designed for the dye decolorization of dyeing wastewater and the degradation of aniline pollutants, acclimates and culturing microorganisms in an environment suitable for each environment in the reaction chambers to make the anaerobic flora They can grow under their optimum environmental conditions, give full play to anaerobic decolorization and degradation, and decolorize the dyes in the process of printing and dyeing wastewater, biodegradation of aromatic compounds such as aniline, and ammonia nitrogen removal in ABR-SBR anaerobic- The aerobic treatment process took place in an orderly manner, so that the printing and dyeing wastewater treatment effect and the stability of the operation were improved, and the overall treatment effect was good. The chromaticity, COD, aniline and other indexes of the treatment system effluent reached the level of the national comprehensive sewage discharge. standard.
The result is to improve the efficiency of wastewater treatment and reduce harmful substances in wastewater. In the treatment of printing and dyeing wastewater, it will become a high-tech printing and dyeing wastewater treatment technology that is economical, practical, safe and has good market prospects.
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