English name: Trypsin (bovine pancreas)
CAS number: 9002-07-7
Molecular weight: 23300 ～ 23800
Grade: USP grade potency: 1: 2500
Vitality: ≥2500 USP U / mg (equal to 10000 BAEE u / mg)
Loss on drying: ≤5.0%
Chymotrypsin: ≤50u / mg
Properties: An endopeptidase extracted from bovine pancreas, white or off-white powder. Soluble in water, insoluble in ethanol, glycerin, chloroform and ether. Isoelectric point pI 10.5. The optimal pH value is about 7.8 ~ 8.5, the pH value is 2 ~ 3 is stable, the pH value above 5 is easy to autodissolve and inactivate, and the pH> 9.0 is irreversible inactivation. Ca2 + has a stabilizing effect on enzyme activity; heavy metal ions, organophosphorus compounds, DFP, and natural trypsin inhibitors have strong inhibitory effects on their activities. It specifically catalyzes the hydrolysis of peptide bonds formed by lysine and arginine carboxyl groups and can be used for protein chemistry research. Storage: 2 ～ 8 ℃
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